As a signature pattern to detect ATPase gamma subunits, a14 residue conserved segment where the last amino acid is found one to three residues from the C-terminal extremity was used. If this is supposed to be your only issue, do not do this. It has been shown [1] that these CDK regulatory subunits assemble as an hexamer which then acts as a hub for the oligomerization of six CDK catalytic subunits. For example, horseradish peroxidase is usually detected by its ability to convert 3,3',5,5'-tetramethylbenzidine TNB to a blue pigment, quantifiable with a spectrophotometer. These untranslated regions may be associated with particular functions such as increasing mRNA message stability.
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