Evaluation of the specificity of primer pairs for detecting highly similar miRNAs. Oxford Academic. Lisheng Zhang. Further, the genetic instability of influenza gene-containing plasmids in bacteria especially Polymerase Basic 2 and Polymerase Basic 1 genes; PB2 and PB1 also leads to erroneous incorporation of bacterial genomic sequences into the influenza gene of interest. Depending on resource availability, it should be feasible to increase sensitivity further by preforming triplicate assays.
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