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The diet had to have at least 0. The enhancement was evident in day-old rats fed the diets for 8 days but not in older rats. McAleese and Forbes found that a diet containing 0. Animals become lethargic and comatose and may die within 3 weeks. Buckingham studied the requirements of rats fed purified diets containing 20 percent lipid with polyunsaturated to saturated P:S ratios of 0.

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